Information om | Engelska ordet IPTG

Exempel på hur man kan använda IPTG i en mening

• The promoters used in expression vector are normally inducible, meaning that protein synthesis is only initiated when required by the introduction of an inducer such as IPTG.
• A non-hydrolyzable analog of allolactose, isopropyl β-D-1-thiogalactopyranoside (IPTG), is normally used in molecular biology to induce the lac operon.
• Like allolactose, IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon, such as the gene coding for beta-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides.
• IPTG is used as an inducer that binds to lac repressor releasing the DNA and allowing transcription.
• Isopropyl β-D-1-thiogalactopyranoside (IPTG), which functions as the inducer of the lac operon, may be used in the media to enhance the expression of LacZ.
• IPTG induced overexpression of kaiA led to arrhythmicity, demonstrating that rhythmicity requires the expression of kaiA as well as the other genes.

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